Improved ROS measurement in root hair cells

Silvina Paola Denita Juárez, Silvina Mangano, José M. Estevez

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

5 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241-1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2',7-dihidro dicloro fluorescein (H2DCFDA).

Original languageEnglish
Title of host publicationPlant Cell Expansion
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York LLC
Pages67-71
Number of pages5
ISBN (Electronic)9781493919024
ISBN (Print)9781493919017
DOIs
Publication statusPublished - 18 Nov 2014

Keywords

  • 2',7-Dihidro dicloro fluorescein
  • Root hair
  • ROS

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine(all)

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