Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in arabidopsis

Francisca Blanco, Virginia Garretón, Nicolas Frey, Calixto Dominguez, Tomás Pérez-Acle, Dominique Van Der Straeten, Xavier Jordana, Loreto Holuigue

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Salicylic acid (SA) plays a crucial role in stress resistance in plants by modifying the expression of a battery of genes. In this paper, we report the identification of a group of early SA-regulated genes of Arabidopsis (activated between 0.5-2.5 h), using the cDNA-amplified fragment length polymorphism technique (cDNA-AFLP). Using 128 different primer combinations, we identified several genes based on their differential expression during SA treatment. Among these, we identified 12 genes up-regulated by SA whose patterns of induction were confirmed by Northern analysis. The identified genes can be grouped into two functional groups: Group 1: genes involved in cell protection (i.e. glycosyltransferases, glutathion S-transferases), and Group 2: genes involved in signal transduction (protein kinases and transcription factors). We also evaluated NPR1 requirement for the induction of the 12 up-regulated genes, and found that only those belonging to Group 2 require this co-activator for their expression. In silico analysis of the promoter sequences of the up-regulated genes, allowed us to identify putative cis-elements over-represented in these genes. Interestingly, as-1-like elements, previously characterized as SA-responsive elements, were specifically over-represented in Group 1 genes. The identification of early SA-regulated genes is an important step towards understanding the complex role of this hormone in plant stress resistance.

Original languageEnglish
Pages (from-to)927-944
Number of pages18
JournalPlant Molecular Biology
Volume59
Issue number6
DOIs
Publication statusPublished - 1 Dec 2005

Keywords

  • As-1
  • cDNA-AFLP
  • Cell protection to stress
  • Early activated genes
  • NPR1
  • Salicylic acid

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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    Blanco, F., Garretón, V., Frey, N., Dominguez, C., Pérez-Acle, T., Van Der Straeten, D., Jordana, X., & Holuigue, L. (2005). Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in arabidopsis. Plant Molecular Biology, 59(6), 927-944. https://doi.org/10.1007/s11103-005-2227-x