Identification of a Renibacterium salmoninarum DNA fragment associated with bacterial internalization into CHSE-cultured cells

Nancy P. Maulén, Pedro J. Morales, Dora Aruti, Jaime E. Figueroa, Margarita I. Concha, Manuel Krauskopf, Gloria León

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

We report here the isolation of a Renibacterium salmoninarum DNA sequence capable of transforming a non-invasive Escherichia coli strain into a microorganism able to enter the fish cell line, CHSE-214. Immunofluorescence and electron microscopy techniques were used to assess the acquired invasive phenotype by HB101 E. coli cells, upon transformation with pPMV-189. This plasmid carries a 2282-bp R. salmoninarum DNA segment. The invasive phenotype is conserved upon deletion of approximately 1000 bp at the 3' end of the insert. The remaining segment contains an ORF region encoding a putative protein of about 30 kDa.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalFEMS Microbiology Letters
Volume135
Issue number1
DOIs
Publication statusPublished - 1 Jan 1996

Keywords

  • Bacterial internalization
  • Bacterial kidney disease
  • Renibacterium salmoninarum

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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