Identification and characterization of GONST1, a Golgi-localized GDP-mannose transporter in arabidopsis

T. C. Baldwin, M. G. Handford, M. I. Yuseff, A. Orellana, P. Dupree

Research output: Contribution to journalArticlepeer-review

129 Citations (Scopus)

Abstract

Transport of nucleotide sugars across the Golgi apparatus membrane is required for the luminal synthesis of a variety of plant cell surface components. We identified an Arabidopsis gene encoding a nucleotide sugar transporter (designated GONST1) that we have shown by transient gene expression to be localized to the Golgi. GONST1 complemented a GDP-mannose transport-defective yeast mutant (vrg4-2), and Golgi-rich vesicles from the complemented strain displayed increased GDP-mannose transport activity. GONST1 promoter::β-glucuronidase studies suggested that this gene is expressed ubiquitously. The identification of a Golgi-localized nucleotide sugar transporter from plants will allow the study of the importance of this class of proteins in the synthesis of plant cell surface components such as cell wall polysaccharides.

Original languageEnglish
Pages (from-to)2283-2295
Number of pages13
JournalPlant Cell
Volume13
Issue number10
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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