HIV-1 mRNA 3′ End Processing Is Distinctively Regulated by eIF3f, CDK11, and Splice Factor 9G8

Susana T. Valente, Greg M. Gilmartin, Krishnan Venkatarama, Gloria Arriagada, Stephen P. Goff

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

A genetic screen previously identified the N-terminal 91 amino acids of the eukaryotic initiation factor 3 subunit f (N91-eIF3f) as a potent inhibitor of HIV-1 replication. Overexpression of N91-eIF3f or full-length eIF3f reduced the level of HIV-1 mRNAs in the infected cell. Here we show that N91-eIF3f and eIF3f act by specifically blocking the 3′ end processing of the HIV-1 pre-mRNA both in vivo and in vitro. Furthermore, the results suggest that eIF3f mediates this restriction of HIV-1 expression through the previously unsuspected involvement of a set of factors that includes eIF3f, the SR protein 9G8, and the cyclin-dependent kinase 11 (CDK11). eIF3f affects HIV-1 3′ end processing by modulating the sequence-specific recognition of the HIV-1 pre-mRNA by 9G8.

Original languageEnglish
Pages (from-to)279-289
Number of pages11
JournalMolecular Cell
Volume36
Issue number2
DOIs
Publication statusPublished - 23 Oct 2009

Keywords

  • HUMDISEASE
  • PROTEINS
  • RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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