Histone deposition and chromatin assembly by RSF

Alejandra Loyola, Danny Reinberg

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

It is becoming clear that the structure of cellular chromatin is dynamic and capable of undergoing rapid changes to respond to the metabolic requirements of the cell. These changes have a direct impact on gene expression and, therefore, the chromatin context must be considered when biochemical reactions that involve DNA are studied. Over the past several decades, a number of methods for assembling chromatin in vitro have been described. Some of them use chemical compounds to deposit histone octamers onto the DNA. Others take advantage of cellular protein complexes that have the ability to assemble chromatin. Some of these complexes have been identified and purified. This article focuses on one of these factors, RSF (remodeling and spacing factor), which was identified in our laboratory. We describe how the chromatin assembly reaction is performed and how it can be monitored to evaluate its efficiency.

Original languageEnglish
Pages (from-to)96-103
Number of pages8
JournalMethods
Volume31
Issue number1
DOIs
Publication statusPublished - 1 Sep 2003

Keywords

  • Chromatin assembly
  • Histones
  • Recombinant chromatin
  • Remodeling and spacing factor (RSF)

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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