High-frequency field stimulation of primary neurons enhances ryanodine receptor-mediated Ca2+ release and generates hydrogen peroxide, which jointly stimulate NF-κB activity

Denise Riquelme, Alvaro Alvarez, Nancy Leal, Tatiana Adasme, Italo Espinoza, Juan Antonio Valdés, Natalia Troncoso, Steffen Hartel, Jorge Hidalgo, Cecilia Hidalgo, M. Angélica Carrasco

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Neuronal electrical activity increases intracellular Ca2+ concentration and generates reactive oxygen species. Here, we show that high frequency field stimulation of primary hippocampal neurons generated Ca 2+ signals with an early and a late component, and promoted hydrogen peroxide generation via a neuronal NADPH oxidase. Hydrogen peroxide generation required both Ca2+ entry through N-methyl-D-aspartate receptors and Ca2+ release mediated by ryanodine receptors (RyR). Field stimulation also enhanced nuclear translocation of the NF-κB p65 protein and NF-κB -dependent transcription, and increased c-fos mRNA and type-2 RyR protein content. Preincubation with inhibitory ryanodine or with the antioxidant N-acetyl L-cysteine abolished the increase in hydrogen peroxide generation and the late Ca2+ signal component induced by electrical stimulation. Primary cortical cells behaved similarly as primary hippocampal cells. Exogenous hydrogen peroxide also activated NF-κB-dependent transcription in hippocampal neurons; inhibitory ryanodine prevented this effect. Selective inhibition of the NADPH oxidase or N-acetyl L-cysteine also prevented the enhanced translocation of p65 in hippocampal cells, while N-acetyl L-cysteine abolished the increase in RyR2 protein content induced by high frequency stimulation. In conclusion, the present results show that electrical stimulation induced reciprocal activation of ryanodine receptor-mediated Ca2+ signals and hydrogen peroxide generation, which stimulated jointly NF-κB activity.

Original languageEnglish
Pages (from-to)1245-1259
Number of pages15
JournalAntioxidants and Redox Signaling
Volume14
Issue number7
DOIs
Publication statusPublished - 1 Apr 2011

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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