Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E.coli phenylalanyl-tRNA1Val on BD-cellulose

Eduardo Rosenmann, Manuel Krauskopf

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The chromatographic behaviour of E.coli tRNA1Val, Val-tRNA1Val and Phe-tRNA1Val was studied on BD-cellulose columns. At pH 4.0 and 4°C the elution position of Phe-tRNA1Val was not affected by the presence or absence of Mg2+, whereas Val-tRNA1Val was slightly retarded when Mg2+ was ommited. It is postulated that the amino acid and its nature influence the structure that the aminoacyl-tRNA assumes. Under suitable conditions the heteroaminoacylated Phe-tRNA1Val eluted significantly later than other tRNAs. This fact showed that heterologous mischarging can be a useful step in tRNA purification methods.

Original languageEnglish
Pages (from-to)935-944
Number of pages10
JournalNucleic Acids Research
Volume1
Issue number8
DOIs
Publication statusPublished - Aug 1974

ASJC Scopus subject areas

  • Genetics

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