Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans

María Cristina Ravanal, Yeison Espinosa, Lorena Rosa, Inmaculada Vaca, Rubén Polanco, Jaime Eyzaguirre, Gloria Levicán, Renato Chávez

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0. 72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.

Original languageEnglish
Pages (from-to)152-155
Number of pages4
JournalMycoscience
Volume53
Issue number2
DOIs
Publication statusPublished - Mar 2012

Keywords

  • Glucose induction
  • Heterologous expression
  • Penicillium purpurogenum
  • Xylanase

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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