TY - JOUR
T1 - Global transcriptomic analysis uncovers a switch to anaerobic metabolism in tellurite-exposed Escherichia coli
AU - Molina-Quiroz, Roberto C.
AU - Loyola, David E.
AU - Díaz-Vásquez, Waldo A.
AU - Arenas, Felipe A.
AU - Urzúa, Ulises
AU - Pérez-Donoso, José M.
AU - Vásquez, Claudio C.
N1 - Publisher Copyright:
© 2014 Institut Pasteur.
PY - 2014
Y1 - 2014
N2 - Tellurite (TeO32-) is harmful for most microorganisms, especially Gram-negative bacteria. Even though tellurite toxicity involves a number of individual aspects, including oxidative stress, malfunctioning of metabolic enzymes and a drop in the reduced thiol pool, among others, the general mechanism of toxicity is rather complex and not completely understood to date. This work focused on DNA microarray analysis to evaluate the Escherichia coli global transcriptomic response when exposed to the toxicant.Confirming previous results, the induction of the oxidative stress response regulator soxS was observed. Upregulation of a number of genes involved in the global stress response, protein folding, redox processes and cell wall organization was also detected. In addition, downregulation of aerobic respiration-related genes suggested a metabolic switch to anaerobic respiration. The expression results were validated through oxygen consumption experiments, which corroborated that tellurite-exposed cells effectively consume oxygen at lower rates than untreated controls.
AB - Tellurite (TeO32-) is harmful for most microorganisms, especially Gram-negative bacteria. Even though tellurite toxicity involves a number of individual aspects, including oxidative stress, malfunctioning of metabolic enzymes and a drop in the reduced thiol pool, among others, the general mechanism of toxicity is rather complex and not completely understood to date. This work focused on DNA microarray analysis to evaluate the Escherichia coli global transcriptomic response when exposed to the toxicant.Confirming previous results, the induction of the oxidative stress response regulator soxS was observed. Upregulation of a number of genes involved in the global stress response, protein folding, redox processes and cell wall organization was also detected. In addition, downregulation of aerobic respiration-related genes suggested a metabolic switch to anaerobic respiration. The expression results were validated through oxygen consumption experiments, which corroborated that tellurite-exposed cells effectively consume oxygen at lower rates than untreated controls.
KW - Anaerobic respiration
KW - Microarrays
KW - Oxidative stress
KW - Tellurite
UR - http://www.scopus.com/inward/record.url?scp=84920036099&partnerID=8YFLogxK
U2 - 10.1016/j.resmic.2014.07.003
DO - 10.1016/j.resmic.2014.07.003
M3 - Article
C2 - 25049169
AN - SCOPUS:84920036099
SN - 0923-2508
VL - 165
SP - 566
EP - 570
JO - Research in Microbiology
JF - Research in Microbiology
IS - 7
ER -