Genome assembly and particle maturation of the birnavirus infectious pancreatic necrosis virus

Rodrigo A. Villanueva, José L. Galaz, Juan A. Valdés, Matilde M. Jashés, Ana María Sandino

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

In this study, we have analyzed the morphogenesis of the birnavirus infectious pancreatic necrosis virus throughout the infective cycle in CHSE-214 cells by using a native agarose electrophoresis system. Two types of viral particles (designated A and B) were identified, isolated, and characterized both molecularly and biologically. Together, our results are consistent with a model of morphogenesis in which the genomic double-stranded RNA is immediately assembled, after synthesis, into a large (66-nm diameter) and uninfectious particle A, where the capsid is composed of both mature and immature viral polypeptides. Upon maturation, particles A yield particles B through the proteolytic cleavage of most of the remaining viral precursors within the capsid, the compaction of the particle (60-nm diameter), and the acquisition of infectivity. These studies will provide the foundation for further analyses of birnavirus particle assembly and RNA replication.

Original languageEnglish
Pages (from-to)13829-13838
Number of pages10
JournalJournal of Virology
Volume78
Issue number24
DOIs
Publication statusPublished - Dec 2004

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint

Dive into the research topics of 'Genome assembly and particle maturation of the birnavirus infectious pancreatic necrosis virus'. Together they form a unique fingerprint.

Cite this