Gene transfer between related bacteria by electrotransformation: Mapping Salmonella typhi genes in Salmonella typhimurium

C. S. Toro, G. C. Mora, N. Figueroa-Bossi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Transfer of newly isolated mutations into a fresh background is an essential step of genetic analysis and strain construction. Gene transfer is hampered in Salmonella typhi and in other pathogenic bacteria by the lack of a generalized transduction system. We show here that this problem can be partially circumvented by using electrotransformation as a means for delivering S. typhi DNA into suitable S. typhi or Salmonella typhimurium recipients. Transferred DNA can recombine with the homologous region in the host chromosome. In one application of the method, mutations isolated in S. typhi were genetically mapped in S. typhimurium.

Original languageEnglish
Pages (from-to)4750-4752
Number of pages3
JournalJournal of Bacteriology
Volume180
Issue number17
DOIs
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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