Gαq negatively regulates the Wnt-β-catenin pathway and dorsal embryonic Xenopus laevis development

Ximena Soto, Roberto Mayor, Marcela Torrejón, Martín Montecino, María Victoria Hinrichs, Juan Olate

Research output: Contribution to journalArticle

7 Citations (Scopus)


The non-canonical Wnt/Ca2+ signaling pathway has been implicated in the regulation of axis formation and gastrulation movements during early Xenopus laevis embryo development, by antagonizing the canonical Wnt/β-catenin dorsalizing pathway and specifying ventral cell fate. However, the molecular mechanisms involved in this antagonist crosstalk are not known. Since Gαq is the main regulator of Ca2+ signaling in vertebrates and from this perspective probably involved in the events elicited by the non-canonical Wnt/Ca2+ pathway, we decided to study the effect of wild-type Xenopus Gq (xGαq) in dorso-ventral axis embryo patterning. Overexpression of xGαq or its endogenous activation at the dorsal animal region of Xenopus embryo both induced a strong ventralized phenotype and inhibited the expression of dorsal-specific mesoderm markers goosecoid and chordin. Dorsal expression of an xGαq dominant-negative mutant reverted the xGαq-induced ventralized phenotype. Finally, we observed that the Wnt8-induced secondary axis formation is reverted by endogenous xGαq activation, indicating that it is negatively regulating the Wnt/β-catenin pathway.

Original languageEnglish
Pages (from-to)483-490
Number of pages8
JournalJournal of Cellular Physiology
Issue number2
Publication statusPublished - Feb 2008

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

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