Frizzled-9 impairs acetylcholine receptor clustering in skeletal muscle cells

Evelyn C. Avilés, Cristina Pinto, Patricia Hanna, Jorge Ojeda, Viviana Pérez, Giancarlo V. De Ferrari, Pedro Zamorano, Miguel Albistur, Daniel Sandoval, Juan P. Henríquez

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Cumulative evidence indicates that Wnt pathways play crucial and diverse roles to assemble the neuromuscular junction (NMJ), a peripheral synapse characterized by the clustering of acetylcholine receptors (AChR) on postsynaptic densities. The molecular determinants of Wnt effects at the NMJ are still to be fully elucidated. We report here that the Wnt receptor Frizzled-9 (Fzd9) is expressed in developing skeletal muscles during NMJ synaptogenesis. In cultured myotubes, gain- and loss-of-function experiments revealed that Fzd9-mediated signaling impairs the AChR-clustering activity of agrin, an organizer of postsynaptic differentiation. Overexpression of Fzd9 induced the cytosolic accumulation of β-catenin, a key regulator of Wnt signaling. Consistently, Fzd9 and β-catenin localize in the postsynaptic domain of embryonic NMJs in vivo. Our findings represent the First evidence pointing to a crucial role of a Fzd-mediated, β-catenin-dependent signaling on the assembly of the vertebrate NMJ.

Original languageEnglish
Article number110
JournalFrontiers in Cellular Neuroscience
Volume8
Issue number1 APR
DOIs
Publication statusPublished - 17 Apr 2014

Keywords

  • Acetylcholine receptor
  • Frizzled receptors
  • Neuromuscular junction
  • Postsynaptic
  • Skeletal muscle
  • Wnt proteins

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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