FlAsH-based live-cell fluorescent imaging of synthetic peptides expressed in Arabidopsis and tobacco

José M. Estévez, Chris Somerville

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter. Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT2 reagents.

Original languageEnglish
Pages (from-to)569-574
Number of pages6
JournalBioTechniques
Volume41
Issue number5
DOIs
Publication statusPublished - 1 Nov 2006
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • General Biochemistry,Genetics and Molecular Biology

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