Fish skeletal muscle tissue is an important focus of immune reactions during pathogen infection

Cristián A. Valenzuela, Rodrigo Zuloaga, Matías Poblete-Morales, Tamara Vera-Tobar, Luis Mercado, Ruben Avendaño-Herrera, Juan Antonio Valdés, Alfredo Molina

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


Skeletal muscle in mammals can express and secrete immune-related molecules during pathogen infection. Despite in fish is known that classical immune tissues participate in innate immunity, the role of skeletal muscle in this function is poorly understood. To determine the immunocompetence of fish skeletal muscle, juvenile fine flounder (Paralichthys adpersus) were challenged with Vibrio ordalii. Different Toll-like receptors, pro-inflammatory cytokines (TNFα, Il-1β, and IL-8), and immune-effector molecules (NKEF and the antimicrobial peptides hepcidin and LEAP-2) were analyzed. Infection initially triggered IL-1β upregulation and P38-MAPK/AP-1 pathway activation. Next, the NFĸB pathway was activated, together with an upregulation of intracellular Toll-like receptor expressions (tlr3, tlr8a tlr9, and tlr21), TNFα production, and leap-2 expression. Finally, transcriptions of il-1β, il-8, tnfα, nkef-a, and hepcidin were also upregulated. These results suggest that fish skeletal muscle is an immunologically active organ that could play an important role against pathogens.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalDevelopmental and Comparative Immunology
Publication statusPublished - 1 Aug 2017


  • Antimicrobial peptides
  • Fish
  • Immune-related genes
  • TLRs
  • V. ordalii

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology


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