Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures

J. W. Swearingen; D. E. Fuentes; M. A. Araya; M. F. Plishker; C. P. Saavedra; T. G. Chasteen; C. C. Vásquez

doi:10.1128/AEM.72.1.963-967.2006

Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures

J. W. Swearingen, D. E. Fuentes, M. A. Araya, M. F. Plishker, C. P. Saavedra, T. G. Chasteen, C. C. Vásquez

Life Sciences School

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide.

Original language	English
Pages (from-to)	963-967
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	1
DOIs	https://doi.org/10.1128/AEM.72.1.963-967.2006
Publication status	Published - Jan 2006

ASJC Scopus subject areas

Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology

Access to Document

10.1128/AEM.72.1.963-967.2006

Cite this

Swearingen, J. W., Fuentes, D. E., Araya, M. A., Plishker, M. F., Saavedra, C. P., Chasteen, T. G., & Vásquez, C. C. (2006). Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures. Applied and Environmental Microbiology, 72(1), 963-967. https://doi.org/10.1128/AEM.72.1.963-967.2006

@article{869e09a8b8c04e8cb92df4951673c563,

title = "Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures",

abstract = "The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide.",

author = "Swearingen, {J. W.} and Fuentes, {D. E.} and Araya, {M. A.} and Plishker, {M. F.} and Saavedra, {C. P.} and Chasteen, {T. G.} and V{\'a}squez, {C. C.}",

year = "2006",

month = jan,

doi = "10.1128/AEM.72.1.963-967.2006",

language = "English",

volume = "72",

pages = "963--967",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "1",

}

Swearingen, JW, Fuentes, DE, Araya, MA, Plishker, MF, Saavedra, CP, Chasteen, TG & Vásquez, CC 2006, 'Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures', Applied and Environmental Microbiology, vol. 72, no. 1, pp. 963-967. https://doi.org/10.1128/AEM.72.1.963-967.2006

Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures. / Swearingen, J. W.; Fuentes, D. E.; Araya, M. A. et al.
In: Applied and Environmental Microbiology, Vol. 72, No. 1, 01.2006, p. 963-967.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures

AU - Swearingen, J. W.

AU - Fuentes, D. E.

AU - Araya, M. A.

AU - Plishker, M. F.

AU - Saavedra, C. P.

AU - Chasteen, T. G.

AU - Vásquez, C. C.

PY - 2006/1

Y1 - 2006/1

N2 - The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide.

AB - The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide.

UR - http://www.scopus.com/inward/record.url?scp=33644862744&partnerID=8YFLogxK

U2 - 10.1128/AEM.72.1.963-967.2006

DO - 10.1128/AEM.72.1.963-967.2006

M3 - Article

C2 - 16391146

AN - SCOPUS:33644862744

SN - 0099-2240

VL - 72

SP - 963

EP - 967

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 1

ER -

Swearingen JW, Fuentes DE, Araya MA, Plishker MF, Saavedra CP, Chasteen TG et al. Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures. Applied and Environmental Microbiology. 2006 Jan;72(1):963-967. doi: 10.1128/AEM.72.1.963-967.2006

Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this