Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta) bajo hidratación y desecación natural

Translated title of the contribution: Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

Loretto Contreras-Porcia, Camilo López-Cristoffanini, Carlos Lovazzano, María Rosa Flores-Molina, Daniela Thomas, Alejandra Núñez, Camila Fierro, Eduardo Guajardo, Juan A. Correa, Michael Kube, Richard Reinhardt

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.

Translated title of the contributionDifferential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
Original languageSpanish
Pages (from-to)933-958
Number of pages26
JournalLatin American Journal of Aquatic Research
Volume41
Issue number5
DOIs
Publication statusPublished - Nov 2013

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science

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    Contreras-Porcia, L., López-Cristoffanini, C., Lovazzano, C., Flores-Molina, M. R., Thomas, D., Núñez, A., Fierro, C., Guajardo, E., Correa, J. A., Kube, M., & Reinhardt, R. (2013). Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta) bajo hidratación y desecación natural. Latin American Journal of Aquatic Research, 41(5), 933-958. https://doi.org/10.3856/vol41-issue5-fulltext-13