TY - JOUR
T1 - Evaluation of functionality of type II toxin-antitoxin systems of Clostridioides difficile R20291
AU - Álvarez, R.
AU - Ortega-Fuentes, C.
AU - Queraltó, C.
AU - Inostroza, O.
AU - Díaz-Yáñez, F.
AU - González, R.
AU - Calderón, I. L.
AU - Fuentes, J. A.
AU - Paredes-Sabja, D.
AU - Gil, F.
N1 - Funding Information:
This work was supported by grants from Agencia Nacional de Investigación y Desarrollo (FONDECYT Grant 1171397 ) to F.G. Funding from the Millennium Science Initiative of the Ministry of Economy, Development, and Tourism , grant “Nucleus in the Biology of Intestinal Microbiota” to D.P-S. and F.G.
Funding Information:
This work was supported by grants from Agencia Nacional de Investigaci?n y Desarrollo (FONDECYT Grant 1171397) to F.G. Funding from the Millennium Science Initiative of the Ministry of Economy, Development, and Tourism, grant ?Nucleus in the Biology of Intestinal Microbiota? to D.P-S. and F.G.
Publisher Copyright:
© 2020 Elsevier GmbH
PY - 2020/10
Y1 - 2020/10
N2 - Clostridioides difficile is a nosocomial, Gram-positive, strictly anaerobic, spore-forming pathogen capable of colonizing and proliferating in the human intestine. In bacteria, it has been shown that the Toxin-Antitoxin systems mediate the cellular response to external stress by initiating processes such as biofilm formation and programmed cell death. This work aims to evaluate the functionality of four type II TA modules of Clostridioides difficile R20291. We performed bioinformatic analysis to search for putative TA systems using the TADB platform. Then we performed a heterologous expression assay to evaluate the functionality of these systems. Our results showed that the MazEF and RelBE systems were functional, suggesting that their corresponding toxins possess an endoribonuclease activity. In conclusion, MazEF and RelBE systems of C. difficile R20291 are functional in a heterologous expression system.
AB - Clostridioides difficile is a nosocomial, Gram-positive, strictly anaerobic, spore-forming pathogen capable of colonizing and proliferating in the human intestine. In bacteria, it has been shown that the Toxin-Antitoxin systems mediate the cellular response to external stress by initiating processes such as biofilm formation and programmed cell death. This work aims to evaluate the functionality of four type II TA modules of Clostridioides difficile R20291. We performed bioinformatic analysis to search for putative TA systems using the TADB platform. Then we performed a heterologous expression assay to evaluate the functionality of these systems. Our results showed that the MazEF and RelBE systems were functional, suggesting that their corresponding toxins possess an endoribonuclease activity. In conclusion, MazEF and RelBE systems of C. difficile R20291 are functional in a heterologous expression system.
KW - Clostridioides difficile
KW - Heterologous expression
KW - Toxin-antitoxin systems
UR - http://www.scopus.com/inward/record.url?scp=85087106498&partnerID=8YFLogxK
U2 - 10.1016/j.micres.2020.126539
DO - 10.1016/j.micres.2020.126539
M3 - Article
AN - SCOPUS:85087106498
SN - 0944-5013
VL - 239
JO - Microbiological Research
JF - Microbiological Research
M1 - 126539
ER -