Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida

Monique Mancuso, Rubén Avendaño-Herrera, R. Zaccone, Alicia E. Toranzo, Beatriz Magariños

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalBiological Research
Volume40
Issue number1
DOIs
Publication statusPublished - 1 Jan 2007

Keywords

  • ERIC-PCR
  • Photobacterium damselae ssp. piscicida
  • RAPD
  • REP-PCR

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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