@article{12ec5a4ad2c7483395e82ef43c9e0262,
title = "ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis",
abstract = "Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.",
author = "Zhaojun Ding and Bangjun Wang and Ignacio Moreno and {Dupl{\'a} Kov{\'a}}, Nikoleta and Sibu Simon and Nicola Carraro and Jesica Reemmer and {P{\v e} N{\v c}{\'i} K}, Ale{\v s} and Xu Chen and Ricardo Tejos and Petr Sk{\AA}pa and Stephan Pollmann and Jozef Mravec and {Petr{\'a} {\v S} Ek}, Jan and Eva Za{\AA}imalova and David Honys and {Rol{\v c}{\'i} K}, Jakub and Angus Murphy and Ariel Orellana and Markus Geisler and Ji{\v r}{\'i} Friml",
note = "Funding Information: We thank Vincent Vincenzetti, Laurence Charrier and Jinsheng Zhu for help with yeast and tobacco transport assays; Mark{\'e}ta Pa{\v r}ezov{\'a} for BY-2 cell line transformations and maintenance; Kl{\'a}ra Hoyerov{\'a} and Petre I. Dobrev for help with metabolic profiling. This work was supported by the funding from the projects CZ.1.07/2.3.00/20.0043 and CZ.1.05/1.1.00/02.0068 (to CEITEC, Central European Institute of Technology) and the Odysseus program of the Research Foundation-Flanders to J.F., from {\textquoteleft}Qilu Scholarship{\textquoteright} of Shandong University (11200081963024) to Z.D., from German Science Foundation (DFG-SFB480/A-10) to S.P., from Swiss National Science Foundation (Grant No. 31003A-125001) and Forschungspool of the University of Fribourg to M.G., from the Ministry of Education, Youth and Sports of the Czech Republic, projects LC06034 to P.S., J.P. and E.Z., KAN200380801 to J.R. (Jakub Rol{\v c}{\'i}k), and OC10054 to N.D., from the Czech Science Foundation (522/09/0858) to D.H, ICM-P10-062-F, Basal Program PFB-16, FONDAP-CRG to A.O. and NSF-IOS (0820648) to A.S.M.",
year = "2012",
doi = "10.1038/ncomms1941",
language = "English",
volume = "3",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
}