ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

Zhaojun Ding, Bangjun Wang, Ignacio Moreno, Nikoleta Duplá Ková, Sibu Simon, Nicola Carraro, Jesica Reemmer, Aleš Pě Nčí K, Xu Chen, Ricardo Tejos, Petr SkÅpa, Stephan Pollmann, Jozef Mravec, Jan Petrá Š Ek, Eva ZaÅimalova, David Honys, Jakub Rolčí K, Angus Murphy, Ariel Orellana, Markus GeislerJiří Friml

Research output: Contribution to journalArticlepeer-review

159 Citations (Scopus)

Abstract

Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.

Original languageEnglish
Article number941
JournalNature Communications
Volume3
DOIs
Publication statusPublished - 2012

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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