TY - JOUR
T1 - Environmental influence on the Atlantic salmon transcriptome and methylome during sea lice infestations
AU - Valenzuela-Muñoz, Valentina
AU - Wanamaker, Shelly
AU - Núñez-Acuña, Gustavo
AU - Roberts, Steven
AU - Garcia, Ana
AU - Valdés, Juan Antonio
AU - Valenzuela-Miranda, Diego
AU - Gallardo-Escárate, Cristian
N1 - Publisher Copyright:
© 2024 Elsevier Ltd
PY - 2024/8
Y1 - 2024/8
N2 - The fish's immune response is affected by different factors, including a wide range of environmental conditions that can also disrupt or promote changes in the host-pathogen interactions. How environmental conditions modulate the salmon genome during parasitism is poorly understood here. This study aimed to explore the environmental influence on the Salmo salar transcriptome and methylome infected with the sea louse Caligus rogercresseyi. Atlantic salmon were experimentally infected with lice at two temperatures (8 and 16 °C) and salinity conditions (32 and 26PSU). Fish tissues were collected from the infected Atlantic salmon for reduced representation bisulfite sequencing (RRBS) and whole transcriptome sequencing (RNA-seq) analysis. The parasitic load was highly divergent in the evaluated environmental conditions, where the lowest lice abundance was observed in fish infected at 8 °C/26PSU. Notably, transcriptome profile differences were statistically associated with the number of alternative splicing events in fish exposed to low temperature/salinity conditions. Furthermore, the temperature significantly affected the methylation level, where high values of differential methylation regions were observed at 16 °C. Also, the association between expression levels of spliced transcripts and their methylation levels was determined, revealing significant correlations with Ferroptosis and TLR KEEG pathways. This study supports the relevance of the environmental conditions during host-parasite interactions in marine ecosystems. The discovery of alternative splicing transcripts associated with DMRs is also discussed as a novel player in fish biology.
AB - The fish's immune response is affected by different factors, including a wide range of environmental conditions that can also disrupt or promote changes in the host-pathogen interactions. How environmental conditions modulate the salmon genome during parasitism is poorly understood here. This study aimed to explore the environmental influence on the Salmo salar transcriptome and methylome infected with the sea louse Caligus rogercresseyi. Atlantic salmon were experimentally infected with lice at two temperatures (8 and 16 °C) and salinity conditions (32 and 26PSU). Fish tissues were collected from the infected Atlantic salmon for reduced representation bisulfite sequencing (RRBS) and whole transcriptome sequencing (RNA-seq) analysis. The parasitic load was highly divergent in the evaluated environmental conditions, where the lowest lice abundance was observed in fish infected at 8 °C/26PSU. Notably, transcriptome profile differences were statistically associated with the number of alternative splicing events in fish exposed to low temperature/salinity conditions. Furthermore, the temperature significantly affected the methylation level, where high values of differential methylation regions were observed at 16 °C. Also, the association between expression levels of spliced transcripts and their methylation levels was determined, revealing significant correlations with Ferroptosis and TLR KEEG pathways. This study supports the relevance of the environmental conditions during host-parasite interactions in marine ecosystems. The discovery of alternative splicing transcripts associated with DMRs is also discussed as a novel player in fish biology.
KW - Alternative splicing
KW - Atlantic salmon
KW - Caligus rogercresseyi
KW - Methylation
KW - Salinity
KW - Temperature
UR - http://www.scopus.com/inward/record.url?scp=85195876654&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2024.109692
DO - 10.1016/j.fsi.2024.109692
M3 - Article
C2 - 38876411
AN - SCOPUS:85195876654
SN - 1050-4648
VL - 151
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
M1 - 109692
ER -