Environmental acclimatization of the carp modulates the transcription of β-actin

José Sarmiento, Sergio Leal, Claudia Quezada, Gudrun Kausel, Jaime Figueroa, María Inés Vera, Manuel Krauskopf

Research output: Contribution to journalArticle

Abstract

A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in β-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential β-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts.

Original languageEnglish
Pages (from-to)223-228
Number of pages6
JournalJournal of Cellular Biochemistry
Volume80
Issue number2
DOIs
Publication statusPublished - 2000

Keywords

  • β-actin transcription
  • Acclimatization
  • Carp
  • Fish
  • Gene expression quantification
  • Housekeeping genes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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