Enhanced synthesis of medium-chain-length poly(3-hydroxyalkanoates) by inactivating the tricarboxylate transport system of Pseudomonas putida KT2440 and process development using waste vegetable oil

José Manuel Borrero-de Acuña, Carla Aravena-Carrasco, Izabook Gutierrez-Urrutia, Daniela Duchens, Ignacio Poblete-Castro

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The use of waste materials as feedstock for biosynthesis of valuable compounds has been an intensive area of research aiming at diminishing the consumption of non-renewable materials. In this study, P. putida KT2440 was employed as a cell factory for the bioconversion of waste vegetable oil into medium-chain-length Polyhydroxyalkanoates. In the presence of the waste oil this environmental strain is capable of secreting enzymes with lipase activities that enhance the bioavailability of this hydrophobic carbon substrate. It was also found that the oxygen transfer coefficient is directly correlated with high PHA levels in KT2440 cells when metabolizing the waste frying oil. By knocking out the tctA gene, encoding for an enzyme of the tripartite carboxylate transport system, an enhanced intracellular level of mcl-PHA was found in the engineered strain when grown on fatty acids. Batch bioreactors showed that the KT2440 strain produced 1.01 (g⋅L−1) of PHA whereas the engineered ΔtctA P. putida strain synthesized 1.91 (g⋅L−1) after 72 h cultivation on 20 (g⋅L−1) of waste oil, resulting in a nearly 2-fold increment in the PHA volumetric productivity. Taken together, this work contributes to accelerate the pace of development for efficient bioconversion of waste vegetable oils into sustainable biopolymers.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalProcess Biochemistry
Volume77
DOIs
Publication statusAccepted/In press - 1 Jan 2018

Keywords

  • Lipase
  • Polyhydroxyalkanoates
  • Pseudomonas putida
  • Tricarboxylate acid transporter
  • Waste vegetable oil

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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