Electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum and chromosomal location of several xylanolytic genes

Renato Chávez, Francisco Fierro, Felipe Gordillo, Juan Francisco Martín, Jaime Eyzaguirre

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum has been resolved. Using contour-clamped homogeneous electric field gel electrophoresis, five chromosomal bands were separated, with estimated sizes of 7.1, 5.2, 3.7, 2.9 and 2.3 Mbp, giving a total genome size of 21.2 Mbp. To our knowledge, this is the smallest Penicillium genome determined so far. By Southern blots and using homologous probes, the chromosomal location of five xylanolytic genes from P. purpurogenum was determined: axeI (acetyl xylan esterase I), xynB (endoxylanase B) and abf1 (arabinofuranosidase 1) in chromosome I, xynA (endoxylanase A) in chromosome II, and axeII (acetyl xylan esterase II) in chromosome III. This is the first study where the location of xylanase genes in a Penicillium genome has been established.

Original languageEnglish
Pages (from-to)379-383
Number of pages5
JournalFEMS Microbiology Letters
Volume205
Issue number2
DOIs
Publication statusPublished - 18 Dec 2001

Keywords

  • Contour-clamped homogeneous electric field
  • Karyotype
  • Penicillium purpurogenum
  • Xylanase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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