Drosophila melanogaster Scramblases modulate synaptic transmission

Usha Acharya, Michael Beth Edwards, Ramon A. Jorquera, Hugo Silva, Kunio Nagashima, Pedro Labarca, Jairaj K. Acharya

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Scramblases are a family of single-pass plasma membrane proteins, identified by their purported ability to scramble phospholipids across the two layers of plasma membrane isolated from platelets and red blood cells. However, their true in vivo role has yet to be elucidated. We report the generation and isolation of null mutants of two Scramblases identified in Drosophila melanogaster. We demonstrate that flies lacking either or both of these Scramblases are not compromised in vivo in processes requiring scrambling of phospho lipids. Instead, we show that D. melanogaster lacking both Scramblases have more vesicles and display enhanced recruitment from a reserve pool of vesicles and increased neurotransmitter secretion at the larval neuromuscular synapses. These defects are corrected by the introduction of a genomic copy of the Scramb 1 gene. The lack of phenotypes related to failure of scrambling and the neurophysiological analysis lead us to propose that Scramblases play a modulatory role in the process of neurotransmission.

Original languageEnglish
Pages (from-to)69-82
Number of pages14
JournalJournal of Cell Biology
Issue number1
Publication statusPublished - 10 Apr 2006
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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