TY - JOUR
T1 - Differential expression of extracellular matrix components in the Fallopian tubes throughout the menstrual cycle
AU - Diaz, Patricia S.
AU - Solar, Paula A.
AU - Juica, Natalia E.
AU - Orihuela, Pedro A.
AU - Cardenas, Hugo
AU - Christodoulides, Myron
AU - Vargas, Renato
AU - Velasquez, Luis A.
N1 - Funding Information:
This study was supported by FONDECYT 1090589, FONDECYT 1120712, Wellcome Trust grant 090301 and Proyecto BASAL FB 0807. The authors acknowledge the writing support of Dr. Horacio Croxatto.
PY - 2012/8/16
Y1 - 2012/8/16
N2 - Background: One of the unique characteristics of the female genital tract is the extensive tissue remodeling observed throughout the menstrual cycle. Multiple components of the extracellular matrix take part in this tissue rebuilding; however, the individual components involved have not been identified.Methods: In the present study, the expression of extracellular matrix proteins and selected matrix metalloproteinase (MMP) activities in Fallopian tubes (FT) throughout the menstrual cycle were examined by PCR array, immunocytochemistry, zymography and bioinformatics.Results: Of the eighty-four genes analyzed, eighty-three were expressed in the FT during at least one stage of the menstrual cycle. We observed a significant increase (>/=2-fold) in ADAMTS1, ADAMTS13, COL7A1, MMP3, MMP9, PECAM1, and THBS3 in the periovulatory phase compared to the follicular phase. Meanwhile, we observed a significant decrease (>/= 2-fold) in COL7A1, ICAM1, ITGA8, MMP16, MMP9, CLEC3B, SELE and TIMP2 in the lutheal phase compared to the periovulatory phase. Immunocytochemistry showed that MMP-3 and MMP-9 were localized in the endosalpinx during all phases of the menstrual cycle. Gelatin zymograms detected non-cycle-dependent protease activity.Conclusions: Several extracellular matrix components were regulated throughout the menstrual cycle in a cyclic pattern, suggesting a possible steroid regulation and a role in tissue remodeling and FT functions.
AB - Background: One of the unique characteristics of the female genital tract is the extensive tissue remodeling observed throughout the menstrual cycle. Multiple components of the extracellular matrix take part in this tissue rebuilding; however, the individual components involved have not been identified.Methods: In the present study, the expression of extracellular matrix proteins and selected matrix metalloproteinase (MMP) activities in Fallopian tubes (FT) throughout the menstrual cycle were examined by PCR array, immunocytochemistry, zymography and bioinformatics.Results: Of the eighty-four genes analyzed, eighty-three were expressed in the FT during at least one stage of the menstrual cycle. We observed a significant increase (>/=2-fold) in ADAMTS1, ADAMTS13, COL7A1, MMP3, MMP9, PECAM1, and THBS3 in the periovulatory phase compared to the follicular phase. Meanwhile, we observed a significant decrease (>/= 2-fold) in COL7A1, ICAM1, ITGA8, MMP16, MMP9, CLEC3B, SELE and TIMP2 in the lutheal phase compared to the periovulatory phase. Immunocytochemistry showed that MMP-3 and MMP-9 were localized in the endosalpinx during all phases of the menstrual cycle. Gelatin zymograms detected non-cycle-dependent protease activity.Conclusions: Several extracellular matrix components were regulated throughout the menstrual cycle in a cyclic pattern, suggesting a possible steroid regulation and a role in tissue remodeling and FT functions.
KW - Extracellular matrix
KW - Fallopian tubes
KW - Menstrual cycle
KW - Metalloproteinases
UR - http://www.scopus.com/inward/record.url?scp=84864962620&partnerID=8YFLogxK
U2 - 10.1186/1477-7827-10-56
DO - 10.1186/1477-7827-10-56
M3 - Article
C2 - 22897899
AN - SCOPUS:84864962620
SN - 1477-7827
VL - 10
JO - Reproductive Biology and Endocrinology
JF - Reproductive Biology and Endocrinology
M1 - 56
ER -