Differences in the endometrial transcript profile during the receptive period between women who were refractory to implantation and those who achieved pregnancy

Alejandro Tapia, Lisa M. Gangi, Fernando Zegers-Hochschild, José Balmaceda, Ricardo Pommer, León Trejo, Isabel Margarita Pacheco, Ana María Salvatierra, Soledad Henríquez, Marisol Quezada, MacArena Vargas, Miguel Ríos, David J. Munroe, Horacio B. Croxatto, Luis Velasquez

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)

Abstract

BACKGROUND: Gene expression profiling of normal receptive endometrium has been characterized, but intrinsic defects in endometrial gene expression associated with implantation failure have not been reported. METHODS: Women who had previously participated as recipients in oocyte donation cycles and repeatedly exhibited implantation failure (Group A, study group) or had at least one successful cycle (Group B, control group) and spontaneously fertile women (Group C, normal fertility group) were recruited. All were treated with exogenous estradiol and progesterone to induce an endometrial cycle, and an endometrial biopsy was taken on the seventh day of progesterone administration. RNA from each sample was analysed by cDNA microarrays to identify differentially expressed genes between groups. RESULTS: 63 transcripts were differentially expressed (≥2-fold) between Groups A and B, of which 16 were subjected to real time RT-PCR. Eleven of these were significantly decreased in Group A with regard to Groups B and C. Among the dysregulated genes were MMP-7, CXCR4, PAEP and C4BPA. CONCLUSIONS: Repeated implantation failure in some oocyte recipients is associated with an intrinsic defect in the expression of multiple genes in their endometrium. Significantly decreased levels of several transcripts in endometria without manifest abnormalities is demonstrated for the first time and shown to be associated with implantation failure.

Original languageEnglish
Pages (from-to)340-351
Number of pages12
JournalHuman Reproduction
Volume23
Issue number2
DOIs
Publication statusPublished - Feb 2008

Keywords

  • Endometrium
  • Implantation
  • Microarrays
  • Oocyte donation
  • Uterine receptivity

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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