Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant

Andrea Rivas-Aravena, Yazmin Fuentes, Julio Cartagena, Tania Brito, Verónica Poggio, José La Torre, Hegaly Mendoza, Fernando Gonzalez-Nilo, Ana María Sandino, Eugenio Spencer

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection.

Original languageEnglish
Pages (from-to)157-166
Number of pages10
JournalFish and Shellfish Immunology
Volume45
Issue number1
DOIs
Publication statusPublished - 1 Jul 2015

Keywords

  • Adjuvant
  • Atlantic salmon
  • ISAV
  • Nanoparticles
  • Salmon alphavirus
  • Vaccine

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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