Detección y cuantificación de sars-cov-2 en plantas de tratamiento de aguas residuales de diferentes ciudades de chile: Hacia la implementación de una vigilancia centinela permanente

Translated title of the contribution: Detection and quantification of sars-cov-2 wastewater treatment plants from different cities in chile; towards to a permanent sentinel surveillance

Jorge Olivares-Pacheco, Aiko D. Adell, Matias I. Hepp, Andressa S. Reis, César Echeverria, Claudia Ibacache-Quiroga, Paulina Assmann, Aldo Gaggero

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Background: The quantification of SARS-CoV-2 in wastewater is a tool that allows determining the trend of viral circulation in a particular geographical area. Aim: To quantify the SARS-CoV-2 virus in 15 wastewater treatment plants in different Chilean cities to establish a comparison with the variables of: I) Active cases per 100,000 inhabitants; ii) daily positivity (novel cases); and iii) phases of the lockdown strategy. Methods: SARS-CoV-2 was concentrated from wastewater samples. To obtain the number of virus genomes per liter, absolute quantification was performed using qRT-PCR. Results: Between January and June 2021, 253 samples were processed, all of which were positive for the presence of the virus. Likewise, it will be determined that the rate of active cases per 100,000 inhabitants is the variable that best fits the trends obtained with the quantification of the viral load in wastewater. Conclusions: The quantification of SARS- CoV-2 in wastewater as a continuous strategy is an efficient tool to determine the trend of the viral circulation in a delimited geographical area and, combined with genomic surveillance, it can constitute an ideal sentinel surveillance alert on future outbreaks.

Translated title of the contributionDetection and quantification of sars-cov-2 wastewater treatment plants from different cities in chile; towards to a permanent sentinel surveillance
Original languageSpanish
Pages (from-to)690-698
Number of pages9
JournalRevista Chilena de Infectologia
Volume39
Issue number6
DOIs
Publication statusPublished - 2022

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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