TY - JOUR
T1 - Defined single-gene and multi-gene deletion mutant collections in salmonella enterica sv typhimurium
AU - Porwollik, Steffen
AU - Santiviago, Carlos A.
AU - Cheng, Pui
AU - Long, Fred
AU - Desai, Prerak
AU - Fredlund, Jennifer
AU - Srikumar, Shabarinath
AU - Silva, Cecilia A.
AU - Chu, Weiping
AU - Chen, Xin
AU - Canals, Rocío
AU - Reynolds, M. Megan
AU - Bogomolnaya, Lydia
AU - Shields, Christine
AU - Cui, Ping
AU - Guo, Jinbai
AU - Zheng, Yi
AU - Endicott-Yazdani, Tiana
AU - Yang, Hee Jeong
AU - Maple, Aimee
AU - Ragoza, Yury
AU - Blondel, Carlos J.
AU - Valenzuela, Camila
AU - Andrews-Polymenis, Helene
AU - McClelland, Michael
PY - 2014/7/9
Y1 - 2014/7/9
N2 - We constructed two collections of targeted single gene deletion (SGD) mutants and two collections of targeted multi-gene deletion (MGD) mutants in Salmonella enterica sv Typhimurium 14028s. The SGD mutant collections contain (1), 3517 mutants in which a single gene is replaced by a cassette containing a kanamycin resistance (KanR) gene oriented in the sense direction (SGD-K), and (2), 3376 mutants with a chloramphenicol resistance gene (Cam R) oriented in the antisense direction (SGD-C). A combined total of 3773 individual genes were deleted across these SGD collections. The MGD collections contain mutants bearing deletions of contiguous regions of three or more genes and include (3), 198 mutants spanning 2543 genes replaced by a KanR cassette (MGD-K), and (4), 251 mutants spanning 2799 genes replaced by a CamR cassette (MGD-C). Overall, 3476 genes were deleted in at least one MGD collection. The collections with different antibiotic markers permit construction of all viable combinations of mutants in the same background. Together, the libraries allow hierarchical screening of MGDs for different phenotypic followed by screening of SGDs within the target MGD regions. The mutants of these collections are stored at BEI Resources (www.beiresources.org) and publicly available.
AB - We constructed two collections of targeted single gene deletion (SGD) mutants and two collections of targeted multi-gene deletion (MGD) mutants in Salmonella enterica sv Typhimurium 14028s. The SGD mutant collections contain (1), 3517 mutants in which a single gene is replaced by a cassette containing a kanamycin resistance (KanR) gene oriented in the sense direction (SGD-K), and (2), 3376 mutants with a chloramphenicol resistance gene (Cam R) oriented in the antisense direction (SGD-C). A combined total of 3773 individual genes were deleted across these SGD collections. The MGD collections contain mutants bearing deletions of contiguous regions of three or more genes and include (3), 198 mutants spanning 2543 genes replaced by a KanR cassette (MGD-K), and (4), 251 mutants spanning 2799 genes replaced by a CamR cassette (MGD-C). Overall, 3476 genes were deleted in at least one MGD collection. The collections with different antibiotic markers permit construction of all viable combinations of mutants in the same background. Together, the libraries allow hierarchical screening of MGDs for different phenotypic followed by screening of SGDs within the target MGD regions. The mutants of these collections are stored at BEI Resources (www.beiresources.org) and publicly available.
UR - http://www.scopus.com/inward/record.url?scp=84904133867&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0099820
DO - 10.1371/journal.pone.0099820
M3 - Article
C2 - 25007190
AN - SCOPUS:84904133867
SN - 1932-6203
VL - 9
JO - PLoS ONE
JF - PLoS ONE
IS - 7
M1 - e99820
ER -