TY - JOUR
T1 - Decrecimiento de streptococcus mutans después de la aplicación de sellantes en superficies oclusales de molares permanentes en adultos
AU - Vildosola, Patricio
AU - Godoy, Eduardo Fernandez
AU - Saad, Jose Roberto Cury
AU - Fluxá, Patricia Palma
AU - Gil, Alain Manuel Chaple
AU - Zepeda, Natalia Acuña
AU - Cortés, Gustavo Moncada
N1 - Publisher Copyright:
© 2019, Editorial Ciencias Medicas. All rights reserved.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Introduction: The main strategy for the prevention of caries disease is the use of pit and fissure sealants, which are indicated for posterior occlusal surfaces that are difficult to clean and cannot be protected very well by fluoride. This strategy is considered to be very important in caries prevention, especially in patients at high cariogenic risk. Objective: Evaluate whether the count of colony forming units of S. mutans per cm2 on the occlusal surface of posterior permanent teeth changes after applying resin-based sealant. Methods: A study was conducted of 38 patients of both genders aged 18-30 years. The patients were at high cariogenic risk and had an indication of occlusal sealant application on at least one permanent posterior tooth. Two samples were taken of dental biofilm from the same teeth before (group T0) and after (group T1) applying sealant obtained by direct impression by tray technique with trypticase yeast extract, cysteine, sucrose with bacitracin (TYCSB), and agar previously made and solidified. The samples were incubated at 37 ºC for 48 hours. The colony forming units (CFU/cm2) were counted. The results were statistically analyzed with the Wilcoxon test at 95 % confidence. Results: Average expression in CFU/cm2 before applying the sealant (T0) and standard deviation was 13.48 (± 14.2), whereas after applying the sealant (T1) it was 5.37 (± 8.90). There was a statistical difference between the two measures T0 and T1 (p< 0.05). Conclusions: Sealant application on occlusal surfaces of posterior permanent teeth significantly reduces the count of CFU/cm2 of S. mutans.
AB - Introduction: The main strategy for the prevention of caries disease is the use of pit and fissure sealants, which are indicated for posterior occlusal surfaces that are difficult to clean and cannot be protected very well by fluoride. This strategy is considered to be very important in caries prevention, especially in patients at high cariogenic risk. Objective: Evaluate whether the count of colony forming units of S. mutans per cm2 on the occlusal surface of posterior permanent teeth changes after applying resin-based sealant. Methods: A study was conducted of 38 patients of both genders aged 18-30 years. The patients were at high cariogenic risk and had an indication of occlusal sealant application on at least one permanent posterior tooth. Two samples were taken of dental biofilm from the same teeth before (group T0) and after (group T1) applying sealant obtained by direct impression by tray technique with trypticase yeast extract, cysteine, sucrose with bacitracin (TYCSB), and agar previously made and solidified. The samples were incubated at 37 ºC for 48 hours. The colony forming units (CFU/cm2) were counted. The results were statistically analyzed with the Wilcoxon test at 95 % confidence. Results: Average expression in CFU/cm2 before applying the sealant (T0) and standard deviation was 13.48 (± 14.2), whereas after applying the sealant (T1) it was 5.37 (± 8.90). There was a statistical difference between the two measures T0 and T1 (p< 0.05). Conclusions: Sealant application on occlusal surfaces of posterior permanent teeth significantly reduces the count of CFU/cm2 of S. mutans.
KW - Composite resins
KW - Permanent dental restoration
KW - Pit and fissure sealants
KW - Streptococcus mutans
UR - http://www.scopus.com/inward/record.url?scp=85077581199&partnerID=8YFLogxK
M3 - Artículo
AN - SCOPUS:85077581199
SN - 0034-7507
VL - 56
JO - Revista Cubana de Estomatologia
JF - Revista Cubana de Estomatologia
IS - 4
ER -