Cystein-serine-rich nuclear protein 1, axud1/Csrnp1, is essential for cephalic neural progenitor proliferation and survival in zebrafish

Carmen G. Feijóo, Andres F. Sarrazin, Miguel L. Allende, Alvaro Glavic

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


The CSRNP (cystein-serine-rich nuclear protein) family has been conserved from Drosophila to human. Although knockout mice for each of the mammalian proteins have been generated, their function during vertebrate development has remained elusive. As an alternative to obtain insights on CSRNP's role in development, we have analysed the expression pattern and function of one member of this family, axud1, during zebrafish development. Our expression analysis indicates that axud1 is expressed from cleavage to larval stages in a dynamic pattern, becoming restricted after gastrulation to anterior regions of the developing neuraxis and later on concentrated predominantly in proliferating domains of the brain. Knockdown analysis using antisense morpholinos shows that reducing Axud1 levels impairs neural progenitor cell proliferation and survival, revealing an essential function of this gene for the growth of cephalic derivatives. The brain growth phenotypes elicited by decreasing Axud1 expression are specific and independent of anterior-posterior patterning events, initial establishment of neural progenitors, or neural differentiation occurring in this tissue. However, Axud1 is necessary for six3.1 expression and is positively regulated by sonic hedgehog. Phylogenetic examination shows that axud1 is likely to be the ortholog of the only member of this family present in Drosophila, as well as to the previously described mouse CSRNP1 and to human AXUD1 (Axin upregulated-1). Thus, we provide evidence as to the role of axud1 in brain growth in vertebrates.

Original languageEnglish
Pages (from-to)2034-2043
Number of pages10
JournalDevelopmental Dynamics
Issue number8
Publication statusPublished - Aug 2008


  • Axud1
  • Brain growth
  • Cell survival
  • Neural progenitors
  • Proliferation

ASJC Scopus subject areas

  • Developmental Biology


Dive into the research topics of 'Cystein-serine-rich nuclear protein 1, axud1/Csrnp1, is essential for cephalic neural progenitor proliferation and survival in zebrafish'. Together they form a unique fingerprint.

Cite this