Clostridium difficile spore biology: Sporulation, germination, and spore structural proteins

Daniel Paredes-Sabja, Aimee Shen, Joseph A. Sorg

Research output: Contribution to journalReview articlepeer-review

199 Citations (Scopus)

Abstract

Clostridium difficile is a Gram-positive, spore-forming obligate anaerobe and a major nosocomial pathogen of worldwide concern. Owing to its strict anaerobic requirements, the infectious and transmissible morphotype is the dormant spore. In susceptible patients, C. difficile spores germinate in the colon to form the vegetative cells that initiate Clostridium difficile infections (CDI). During CDI, C. difficile induces a sporulation pathway that produces more spores; these spores are responsible for the persistence of C. difficile in patients and horizontal transmission between hospitalized patients. Although important to the C. difficile lifecycle, the C. difficile spore proteome is poorly conserved when compared to members of the Bacillus genus. Further, recent studies have revealed significant differences between C. difficile and Bacillus subtilis at the level of sporulation, germination, and spore coat and exosporium morphogenesis. In this review, the regulation of the sporulation and germination pathways and the morphogenesis of the spore coat and exosporium will be discussed.

Original languageEnglish
Pages (from-to)406-416
Number of pages11
JournalTrends in Microbiology
Volume22
Issue number7
DOIs
Publication statusPublished - Jul 2014

Keywords

  • C. difficile spores
  • Exosporium
  • Germination
  • Spore coat
  • Sporulation

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)
  • Infectious Diseases
  • Virology

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