Cloning the BstVI restriction-modification system in Escherichia coli

Claudio Vásquez, Claudia Saavedra, Enrique González

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A standard DNA modification methyltransferase (MTase) selection protocol was followed to clone the BstVI restriction and modification system from Bacillus stearothermophilus in Escherichia coli. Both genes were contained in a 4.4-kb EcoRI fragment from B. stearothermophilus V chromosomal DNA. The heterologous expression of these genes did not depend on their orientation in the vector, suggesting that the genes are expressed in E. coli under the control of promoters located on the cloned fragment. Subcloning experiments demonstrated that the bstVIR gene was expressed in the absence of its cognate MTase.

Original languageEnglish
Pages (from-to)83-85
Number of pages3
JournalGene
Volume102
Issue number1
DOIs
Publication statusPublished - 15 Jun 1991

Keywords

  • Bacillus stearothermophilus
  • Restriction endonuclease
  • heterologous gene expression
  • modification methyltransferase
  • recombinant DNA

ASJC Scopus subject areas

  • Genetics

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