Cloning, sequencing and expression of the cDNA of endoxylanase B from Penicillium purpurogenum

René Díaz, Amalia Sapag, Alessandra Peirano, Jeannette Steiner, Jaime Eyzaguirre

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The cDNA for xylanase B from Penicillium purpurogenum was cloned and sequenced. This DNA encodes a protein of 208 amino acids which is expected to yield a protein of 183 residues upon processing of the N terminus. The sequence of the predicted protein is very similar to that of 40 other xylanase domains which belong to family G of cellulases/xylanases (73-21% identity).

Original languageEnglish
Pages (from-to)247-251
Number of pages5
JournalGene
Volume187
Issue number2
DOIs
Publication statusPublished - 18 Mar 1997

Keywords

  • Codon usage
  • Endoxylanase
  • Glycosyl hydrolase
  • Homology comparison
  • Nucleotide sequence
  • P. purpurogenum
  • PCR
  • Sequence alignment

ASJC Scopus subject areas

  • Genetics

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