Cloning of a Thiobacillus ferrooxidans plasmid in Escherichia coli

D. S. Holmes, J. H. Lobos, L. H. Bopp, G. C. Welch

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Three separate plasmids of 6, 7, 16, and > 23 kilobases were purified from a single clone of Thiobacillus ferrooxidans ATCC 33020 grown in the presence of uranium. The 6.7-kilobase plasmid (pTf1) was cloned separately into the HindIII or BamHI site of Escherichia coli plasmid pBR322. Restriction maps of the recombinant plasmids, termed pTf100 and pTf110, respectively, were constructed, creating potential cloning vehicles for exchanging genetic information between E. coli and T. ferrooxidans. Evidence from restriction enzyme analysis and Southern blot DNA-DNA hybridization indicates that the three native plasmids share little consequence homology.

Original languageEnglish
Pages (from-to)324-326
Number of pages3
JournalJournal of Bacteriology
Volume157
Issue number1
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Molecular Biology

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