Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli

J. Agüero, G. Mora, M. J. Mroczenski-Wildey, M. E. Fernandez-Beros, L. Aron, F. C. Cabello

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

A recombinant plasmid containing the gene for the 36 KDal porin of Salmonella typhi has been identified in a cosmid library of S. typhi propagated in Escherichia coli. The recombinant clone was identified by its ability to endow E. coli with susceptibility to porin specific phages, and by the appearance in the outer membrane of E. coli containing the clone of a new protein of 36 KDal. While the porin confers upon a porinless mutant of E. coli an increased susceptibility to β-lactam antibiotics, it does not react with serum from patients with typhoid fever in immunoblotting assays.

Original languageEnglish
Pages (from-to)399-407
Number of pages9
JournalMicrobial Pathogenesis
Volume3
Issue number6
DOIs
Publication statusPublished - 1 Jan 1987

Keywords

  • 36 KDal porin
  • cloning in E. coli
  • immunoblotting
  • Salmonella typhi
  • typhoid fever

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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