Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli

J. Agüero; G. Mora; M. J. Mroczenski-Wildey; M. E. Fernandez-Beros; L. Aron; F. C. Cabello

doi:10.1016/0882-4010(87)90010-6

Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli

J. Agüero, G. Mora, M. J. Mroczenski-Wildey, M. E. Fernandez-Beros, L. Aron, F. C. Cabello

Medicine School

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A recombinant plasmid containing the gene for the 36 KDal porin of Salmonella typhi has been identified in a cosmid library of S. typhi propagated in Escherichia coli. The recombinant clone was identified by its ability to endow E. coli with susceptibility to porin specific phages, and by the appearance in the outer membrane of E. coli containing the clone of a new protein of 36 KDal. While the porin confers upon a porinless mutant of E. coli an increased susceptibility to β-lactam antibiotics, it does not react with serum from patients with typhoid fever in immunoblotting assays.

Original language	English
Pages (from-to)	399-407
Number of pages	9
Journal	Microbial Pathogenesis
Volume	3
Issue number	6
DOIs	https://doi.org/10.1016/0882-4010(87)90010-6
Publication status	Published - 1 Jan 1987

Keywords

36 KDal porin
cloning in E. coli
immunoblotting
Salmonella typhi
typhoid fever

ASJC Scopus subject areas

Microbiology
Infectious Diseases

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1016/0882-4010(87)90010-6

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title = "Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli",

abstract = "A recombinant plasmid containing the gene for the 36 KDal porin of Salmonella typhi has been identified in a cosmid library of S. typhi propagated in Escherichia coli. The recombinant clone was identified by its ability to endow E. coli with susceptibility to porin specific phages, and by the appearance in the outer membrane of E. coli containing the clone of a new protein of 36 KDal. While the porin confers upon a porinless mutant of E. coli an increased susceptibility to β-lactam antibiotics, it does not react with serum from patients with typhoid fever in immunoblotting assays.",

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T1 - Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli

AU - Agüero, J.

AU - Mora, G.

AU - Mroczenski-Wildey, M. J.

AU - Fernandez-Beros, M. E.

AU - Aron, L.

AU - Cabello, F. C.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - A recombinant plasmid containing the gene for the 36 KDal porin of Salmonella typhi has been identified in a cosmid library of S. typhi propagated in Escherichia coli. The recombinant clone was identified by its ability to endow E. coli with susceptibility to porin specific phages, and by the appearance in the outer membrane of E. coli containing the clone of a new protein of 36 KDal. While the porin confers upon a porinless mutant of E. coli an increased susceptibility to β-lactam antibiotics, it does not react with serum from patients with typhoid fever in immunoblotting assays.

AB - A recombinant plasmid containing the gene for the 36 KDal porin of Salmonella typhi has been identified in a cosmid library of S. typhi propagated in Escherichia coli. The recombinant clone was identified by its ability to endow E. coli with susceptibility to porin specific phages, and by the appearance in the outer membrane of E. coli containing the clone of a new protein of 36 KDal. While the porin confers upon a porinless mutant of E. coli an increased susceptibility to β-lactam antibiotics, it does not react with serum from patients with typhoid fever in immunoblotting assays.

KW - 36 KDal porin

KW - cloning in E. coli

KW - immunoblotting

KW - Salmonella typhi

KW - typhoid fever

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Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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