Cloning and functional characterization of the gene encoding the transcription factor Ace1 in the basidiomycete Phanerochaete chrysosporium

Rubén Polanco, Paulo Canessa, Alexis Rivas, Luis F. Larrondo, Sergio Lobos, Rafael Vicuña

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-ace1 encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Ace1, Mac1 and Haa1 from Saccharomyces cerevisiae. The Pc-ace1 gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae ace1 null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-ace 1. Moreover, Northern blot hybridization studies indicated that Pc-ace1 cDNA restores copper inducibility of the yeast cup1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Ace1 transcription factor in basidiomycetes.

Original languageEnglish
Pages (from-to)641-648
Number of pages8
JournalBiological Research
Volume39
Issue number4
Publication statusPublished - 2006

Keywords

  • Ace1
  • Basidiomycete
  • Copper
  • Phanerochaete chrysosporium

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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