Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line

Y. Shaul, M. Ziemer, P. D. Garcia, R. Crawford, H. Hsu, P. Valenzuela, W. J. Rutter

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We report the isolation by molecular cloning and the analysis by heteroduplex and restriction enzyme mapping of seven distinct DNA fragments containing hepatitis B virus (HBV) sequences from genomic DNA of the PLC/PRF/5 human liver carcinoma cell line (the Alexander cell). No intact full-length HBV genomes were present. Three different patterns of organization of HBV fragments were detected. These included two linear fragments without detectable rearrangement, three other HBV fragments with internal deletions, and two HBV fragments containing long inverted duplications. HBsAg sequences are preferentially included in the integrated fragment, whereas the core gene is preferentially eliminated. Several of the integrated HBV fragments might act as templates for the synthesis of functional HBsAg mRNA, whereas only one clone could produce a full core antigen transcript.

Original languageEnglish
Pages (from-to)776-787
Number of pages12
JournalJournal of Virology
Volume51
Issue number3
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line'. Together they form a unique fingerprint.

  • Cite this

    Shaul, Y., Ziemer, M., Garcia, P. D., Crawford, R., Hsu, H., Valenzuela, P., & Rutter, W. J. (1984). Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line. Journal of Virology, 51(3), 776-787.