Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana

Eliana Marzol, Cecilia Borassi, Mariana Carignani Sardoy, Philippe Ranocha, Ariel A. Aptekmann, Mauro Bringas, Janice Pennington, Julio Paez-Valencia, Javier Martínez Pacheco, Diana R. Rodríguez-Garcia, Yossmayer Del Carmen Rondón Guerrero, Juan Manuel Peralta, Margaret Fleming, John W. Mishler-Elmore, Silvina Mangano, Francisca Blanco-Herrera, Patricia A. Bedinger, Christophe Dunand, Luciana Capece, Alejandro D. NadraMichael Held, Marisa S. Otegui, José M. Estevez

Research output: Contribution to journalArticlepeer-review

Abstract

Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. prx01,44,73 triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in prx01,44,73 and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells.

Original languageEnglish
Article number5375
JournalInternational Journal of Molecular Sciences
Volume23
Issue number10
DOIs
Publication statusPublished - 2 May 2022

Keywords

  • Arabidopsis
  • cell walls
  • class-III peroxidases
  • extensins
  • root hairs
  • ROS

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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