Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana

Eliana Marzol; Cecilia Borassi; Mariana Carignani Sardoy; Philippe Ranocha; Ariel A. Aptekmann; Mauro Bringas; Janice Pennington; Julio Paez-Valencia; Javier Martínez Pacheco; Diana R. Rodríguez-Garcia; Yossmayer Del Carmen Rondón Guerrero; Juan Manuel Peralta; Margaret Fleming; John W. Mishler-Elmore; Silvina Mangano; Francisca Blanco-Herrera; Patricia A. Bedinger; Christophe Dunand; Luciana Capece; Alejandro D. Nadra; Michael Held; Marisa S. Otegui; José M. Estevez

doi:10.3390/ijms23105375

Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana

Eliana Marzol, Cecilia Borassi, Mariana Carignani Sardoy, Philippe Ranocha, Ariel A. Aptekmann, Mauro Bringas, Janice Pennington, Julio Paez-Valencia, Javier Martínez Pacheco, Diana R. Rodríguez-Garcia, Yossmayer Del Carmen Rondón Guerrero, Juan Manuel Peralta, Margaret Fleming, John W. Mishler-Elmore, Silvina Mangano, Francisca Blanco-Herrera, Patricia A. Bedinger, Christophe Dunand, Luciana Capece, Alejandro D. NadraMichael Held, Marisa S. Otegui, José M. Estevez

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20 Citations (Scopus)

Abstract

Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. prx01,44,73 triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in prx01,44,73 and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells.

Original language	English
Article number	5375
Journal	International Journal of Molecular Sciences
Volume	23
Issue number	10
DOIs	https://doi.org/10.3390/ijms23105375
Publication status	Published - 2 May 2022

Keywords

Arabidopsis
cell walls
class-III peroxidases
extensins
root hairs
ROS

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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10.3390/ijms23105375

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Marzol, E., Borassi, C., Sardoy, M. C., Ranocha, P., Aptekmann, A. A., Bringas, M., Pennington, J., Paez-Valencia, J., Pacheco, J. M., Rodríguez-Garcia, D. R., Guerrero, Y. D. C. R., Peralta, J. M., Fleming, M., Mishler-Elmore, J. W., Mangano, S., Blanco-Herrera, F., Bedinger, P. A., Dunand, C., Capece, L., ... Estevez, J. M. (2022). Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana. International Journal of Molecular Sciences, 23(10), Article 5375. https://doi.org/10.3390/ijms23105375

@article{f01f6c9ee5604de6946bc6b001076c6d,

title = "Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana",

abstract = "Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. prx01,44,73 triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in prx01,44,73 and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells.",

keywords = "Arabidopsis, cell walls, class-III peroxidases, extensins, root hairs, ROS",

author = "Eliana Marzol and Cecilia Borassi and Sardoy, {Mariana Carignani} and Philippe Ranocha and Aptekmann, {Ariel A.} and Mauro Bringas and Janice Pennington and Julio Paez-Valencia and Pacheco, {Javier Mart{\'i}nez} and Rodr{\'i}guez-Garcia, {Diana R.} and Guerrero, {Yossmayer Del Carmen Rond{\'o}n} and Peralta, {Juan Manuel} and Margaret Fleming and Mishler-Elmore, {John W.} and Silvina Mangano and Francisca Blanco-Herrera and Bedinger, {Patricia A.} and Christophe Dunand and Luciana Capece and Nadra, {Alejandro D.} and Michael Held and Otegui, {Marisa S.} and Estevez, {Jos{\'e} M.}",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = may,

day = "2",

doi = "10.3390/ijms23105375",

language = "English",

volume = "23",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

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Marzol, E, Borassi, C, Sardoy, MC, Ranocha, P, Aptekmann, AA, Bringas, M, Pennington, J, Paez-Valencia, J, Pacheco, JM, Rodríguez-Garcia, DR, Guerrero, YDCR, Peralta, JM, Fleming, M, Mishler-Elmore, JW, Mangano, S, Blanco-Herrera, F, Bedinger, PA, Dunand, C, Capece, L, Nadra, AD, Held, M, Otegui, MS & Estevez, JM 2022, 'Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana', International Journal of Molecular Sciences, vol. 23, no. 10, 5375. https://doi.org/10.3390/ijms23105375

TY - JOUR

T1 - Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana

AU - Marzol, Eliana

AU - Borassi, Cecilia

AU - Sardoy, Mariana Carignani

AU - Ranocha, Philippe

AU - Aptekmann, Ariel A.

AU - Bringas, Mauro

AU - Pennington, Janice

AU - Paez-Valencia, Julio

AU - Pacheco, Javier Martínez

AU - Rodríguez-Garcia, Diana R.

AU - Guerrero, Yossmayer Del Carmen Rondón

AU - Peralta, Juan Manuel

AU - Fleming, Margaret

AU - Mishler-Elmore, John W.

AU - Mangano, Silvina

AU - Blanco-Herrera, Francisca

AU - Bedinger, Patricia A.

AU - Dunand, Christophe

AU - Capece, Luciana

AU - Nadra, Alejandro D.

AU - Held, Michael

AU - Otegui, Marisa S.

AU - Estevez, José M.

PY - 2022/5/2

Y1 - 2022/5/2

N2 - Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. prx01,44,73 triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in prx01,44,73 and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells.

AB - Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73. prx01,44,73 triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in prx01,44,73 and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells.

KW - Arabidopsis

KW - cell walls

KW - class-III peroxidases

KW - extensins

KW - root hairs

KW - ROS

UR - http://www.scopus.com/inward/record.url?scp=85129738061&partnerID=8YFLogxK

U2 - 10.3390/ijms23105375

DO - 10.3390/ijms23105375

M3 - Article

AN - SCOPUS:85129738061

SN - 1661-6596

VL - 23

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 10

M1 - 5375

ER -

Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana

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