Characterization of the major plasma apoliproteins of the high density lipoprotein in the carp (Cyprinus carpio)

Rodolfo Amthauer, Julieta Villanueva, Maria Ines Vera, Margarita Concha, Manuel Krauskopf

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

1. 1. Carp plasma DHL was isolated by a single chromatographic procedure using Affi-Gel Blue. Neither LDL nor VLDL were detected. 2. 2. Thus, HDL which comprises almost 40% of the total carp plasma proteins, also constitutes the major lipoprotein fraction in this fish, yielding two apolipoproteins: apo A-I and apo A-II with molecular weight of 29,500 and 12,000, respectively. These are present in a one to one molar ratio. 3. 3. Carp apo A-I and apo A-II lack cysteine and tryptophan. Although some similarities in the amino acid composition exist with respect to the same apoproteins from other organisms, antisera raised against carp apo-I cross-reacted only, and very slightly, with its rainbow trout counterpart. 4. 4. No reaction was detected with lamprey and the nothotenidae robalo fish. 5. 5. Four plasmatic apo A-I isoforms were identified. No differences were observed in carps acclimatized to winter and summer.

Original languageEnglish
Pages (from-to)787-793
Number of pages7
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume92
Issue number4
DOIs
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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