Characterization of synthetic hydroxyproline-rich proteoglycans with arabinogalactan protein and extensin motifs in arabidopsis

José M. Estévez, Marcia J. Kieliszewski, Natalie Khitrov, Chris Somerville

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)

Abstract

A series of gene constructs encoding synthetic glycomodule peptides with N-terminal signal sequences and C-terminal green fluorescent proteins were expressed in transgenic Arabidopsis (Arabidopsis thaliana) under the control of the 35S promoter. The synthetic glycomodule peptides were composed of repetitive proline-containing motifs that have been previously found to be substrates for prolyl hydroxylases and subsequent O-glycosylation of the hydroxyproline residues. All of the constructs were secreted in aerial tissues, but not in roots. The amount of hydroxylation and glycosylation of the various constructs varied depending on the tissue. Also, accumulation of the proteins exhibited a high degree of cell-type specificity within various tissues due to posttranscriptional effects. The observations reveal a high level of complexity in the synthesis, secretion, and turnover of the glycoproteins.

Original languageEnglish
Pages (from-to)458-470
Number of pages13
JournalPlant Physiology
Volume142
Issue number2
DOIs
Publication statusPublished - 1 Oct 2006
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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