Characterization of an agarophyton chilense oleoresin containing pparγ natural ligands with insulin-sensitizing effects in a c57bl/6j mouse model of diet-induced obesity and antioxidant activity in caenorhabditis elegans

Claudio Pinto, María Raquel Ibáñez, Gloria Loyola, Luisa León, Yasmin Salvatore, Carla González, Víctor Barraza, Francisco Castañeda, Rebeca Aldunate, Loretto Contreras-Porcia, Karen Fuenzalida, Francisca C. Bronfman

Research output: Contribution to journalArticlepeer-review

Abstract

The biomedical potential of the edible red seaweed Agarophyton chilense (formerly Gracilaria chilensis) has not been explored. Red seaweeds are enriched in polyunsaturated fatty acids and eicosanoids, which are known natural ligands of the PPARγ nuclear receptor. PPARγ is the molecular target of thiazolidinediones (TZDs), drugs used as insulin sensitizers to treat type 2 diabetes mellitus. Medical use of TZDs is limited due to undesired side effects, a problem that has triggered the search for selective PPARγ modulators (SPPARMs) without the TZD side effects. We produced Agarophyton chilense oleoresin (Gracilex®), which induces PPARγ activation without in-ducing adipocyte differentiation, similar to SPPARMs. In a diet-induced obesity model of male mice, we showed that treatment with Gracilex® improves insulin sensitivity by normalizing altered glucose and insulin parameters. Gracilex® is enriched in palmitic acid, arachidonic acid, oleic acid, and lipophilic antioxidants such as tocopherols and β-carotene. Accordingly, Gracilex® possesses antioxidant activity in vitro and increased antioxidant capacity in vivo in Caenorhabditis elegans. These findings support the idea that Gracilex® represents a good source of natural PPARγ ligands and antioxidants with the potential to mitigate metabolic disorders. Thus, its nutraceutical value in humans warrants further investigation.

Original languageEnglish
Article number1828
JournalNutrients
Volume13
Issue number6
DOIs
Publication statusPublished - Jun 2021

Keywords

  • Agarophyton chilense
  • Anti-oxidants
  • Caenorhabditis elegans
  • Gracilex®
  • Insulin resistance
  • Natural lipids
  • Nutraceuticals
  • Obesity
  • PPARγ
  • Seaweeds

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Fingerprint

Dive into the research topics of 'Characterization of an agarophyton chilense oleoresin containing pparγ natural ligands with insulin-sensitizing effects in a c57bl/6j mouse model of diet-induced obesity and antioxidant activity in caenorhabditis elegans'. Together they form a unique fingerprint.

Cite this