Characteristics of monoclonal antibodies against Piscirickettsia salmonis

A. Jamett, J. Aguayo, A. Miquel, I. Muller, R. Arriagada, M. I. Becker, P. Valenzuela, L. O. Burzio

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

A panel of 28 monoclonal antibodies against Piscirickettsia salmonis was produced using a purified fraction of the bacterium. To determine their specificity to the pathogen, the antibodies were assayed by ELISA and indirect immunofluorescence microscopy. Six monoclonal antibodies were selected based on their strong reaction against P. salmonis and absence of cross-reactivity with other common fish pathogens. Western blot analysis showed that the antibodies reacted to several antigens of P. salmonis. Immunofluorescence assays revealed that these antibodies reacted with the same specificity to different isolates of P. salmonis obtained from the south of Chile. This panel of monoclonal antibodies represents an important tool to develop simple, rapid, sensitive and highly specific methods for the detection of the pathogen and diagnosis of the disease.

Original languageEnglish
Pages (from-to)205-215
Number of pages11
JournalJournal of Fish Diseases
Volume24
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • Immunofluorescence
  • Monoclonal antibodies
  • Piscirickettsia salmonis
  • Piscirickettsiosis
  • Salmon

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary(all)
  • veterinary (miscalleneous)

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