TY - JOUR
T1 - Cell wall calcium and hemicellulose have a role in the fruit firmness during storage of blueberry (Vaccinium spp.)
AU - Olmedo, Patricio
AU - Zepeda, Baltasar
AU - Rojas, Bárbara
AU - Silva-Sanzana, Christian
AU - Delgado-Rioseco, Joaquín
AU - Fernández, Kamila
AU - Balic, Iván
AU - Arriagada, César
AU - Moreno, Adrián A.
AU - Defilippi, Bruno G.
AU - Campos-Vargas, Reinaldo
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/3
Y1 - 2021/3
N2 - The firmness of blueberry is one of its most significant quality attributes. Modifications in the composition of the cell wall have been associated with changes in the fruit firmness. In this work, cell wall components and calcium concentration in two blueberry cultivars with contrasting firmness phenotypes were evaluated at harvest and 30 days cold storage (0◦ C). High performance anion-exchange chromatography with pulse amperometric detector (HPAEC-PAD) analysis was performed using the “Emerald” (firmer) and “Jewel” (softer) blueberry cultivars, showing increased glucose in the firmer cultivar after cold storage. Moreover, the LM15 antibody, which recognizes xyloglucan domains, displayed an increased signal in the Emerald cultivar after 30 d cold storage. Additionally, the antibody 2F4, recognizing a homogalacturonan calcium-binding domain, showed a greater signal in the firmer Emerald blueberries, which correlates with a higher calcium concentration in the cell wall. These findings suggest that xyloglucan metabolism and a higher concentration of cell wall calcium influenced the firmness of the blueberry fruit. These results open new perspectives regarding the role of cell wall components as xyloglucans and calcium in blueberry firmness.
AB - The firmness of blueberry is one of its most significant quality attributes. Modifications in the composition of the cell wall have been associated with changes in the fruit firmness. In this work, cell wall components and calcium concentration in two blueberry cultivars with contrasting firmness phenotypes were evaluated at harvest and 30 days cold storage (0◦ C). High performance anion-exchange chromatography with pulse amperometric detector (HPAEC-PAD) analysis was performed using the “Emerald” (firmer) and “Jewel” (softer) blueberry cultivars, showing increased glucose in the firmer cultivar after cold storage. Moreover, the LM15 antibody, which recognizes xyloglucan domains, displayed an increased signal in the Emerald cultivar after 30 d cold storage. Additionally, the antibody 2F4, recognizing a homogalacturonan calcium-binding domain, showed a greater signal in the firmer Emerald blueberries, which correlates with a higher calcium concentration in the cell wall. These findings suggest that xyloglucan metabolism and a higher concentration of cell wall calcium influenced the firmness of the blueberry fruit. These results open new perspectives regarding the role of cell wall components as xyloglucans and calcium in blueberry firmness.
KW - Calcium
KW - Fruit
KW - Texture
KW - Xyloglucan
UR - http://www.scopus.com/inward/record.url?scp=85102503913&partnerID=8YFLogxK
U2 - 10.3390/plants10030553
DO - 10.3390/plants10030553
M3 - Article
AN - SCOPUS:85102503913
VL - 10
SP - 1
EP - 13
JO - Plants
JF - Plants
SN - 2223-7747
IS - 3
M1 - 553
ER -