TY - JOUR
T1 - C/EBPβ binds the P1 promoter of the Runx2 gene and up-regulates Runx2 transcription in osteoblastic cells
AU - Henriquez, Berta
AU - Hepp, Matias
AU - Merino, Paola
AU - Sepulveda, Hugo
AU - van Wijnen, Andre J.
AU - Lian, Jane B.
AU - Stein, Gary S.
AU - Stein, Janet L.
AU - Montecino, Martin
PY - 2011/11
Y1 - 2011/11
N2 - The Runx2 factor is an essential component of the regulatory mechanisms that control transcription during skeletogenesis. Runx2/p57 expression in osteoblastic cells is controlled by the P1 promoter, which is recognized by key regulators of osteoblast differentiation including homeodomain factors and Wnt- and BMP-signaling mediators. Here, we report that the transcription factor C/EBPβ up-regulates Runx2/p57 expression by directly binding to the Runx2 P1 promoter in mesenchymal, pre-osteoblastic, and osteoblastic cells. This C/EBPβ-mediated up-regulation is principally dependent on C/EBP site II that is located within the first 180bp of the proximal P1 promoter region and is highly conserved among mouse, rat, and human Runx2 genes. Our studies reveal how the C/EBPβ factor, known to have a key role during osteogenesis, contributes to regulating the expression of Runx2, the master regulator of osteoblast differentiation.
AB - The Runx2 factor is an essential component of the regulatory mechanisms that control transcription during skeletogenesis. Runx2/p57 expression in osteoblastic cells is controlled by the P1 promoter, which is recognized by key regulators of osteoblast differentiation including homeodomain factors and Wnt- and BMP-signaling mediators. Here, we report that the transcription factor C/EBPβ up-regulates Runx2/p57 expression by directly binding to the Runx2 P1 promoter in mesenchymal, pre-osteoblastic, and osteoblastic cells. This C/EBPβ-mediated up-regulation is principally dependent on C/EBP site II that is located within the first 180bp of the proximal P1 promoter region and is highly conserved among mouse, rat, and human Runx2 genes. Our studies reveal how the C/EBPβ factor, known to have a key role during osteogenesis, contributes to regulating the expression of Runx2, the master regulator of osteoblast differentiation.
UR - http://www.scopus.com/inward/record.url?scp=79960351659&partnerID=8YFLogxK
U2 - 10.1002/jcp.22652
DO - 10.1002/jcp.22652
M3 - Article
C2 - 21302301
AN - SCOPUS:79960351659
SN - 0021-9541
VL - 226
SP - 3043
EP - 3052
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 11
ER -