CdsH contributes to the replication of salmonella typhimurium inside epithelial cells in a cysteine-supplemented medium

Fernando Díaz-Yáñez, Ricardo Álvarez, Iván L. Calderón, Juan A. Fuentes, Fernando Gil

Research output: Contribution to journalArticlepeer-review

Abstract

Salmonella Typhimurium is a facultative, intracellular pathogen whose products range from self-limited gastroenteritis to systemic diseases. Food ingestion increases biomolecules’ concentration in the intestinal lumen, including amino acids such as cysteine, which is toxic in a concentration-dependent manner. When cysteine’s intracellular concentration reaches toxic levels, S. Typhimurium expresses a cysteine-inducible enzyme (CdsH), which converts cysteine into pyruvate, sulfide, and ammonia. Despite this evidence, the biological context of cdsH’s role is not completely clear, especially in the infective cycle. Since inside epithelial cells both cdsH and its positive regulator, ybaO, are overexpressed, we hypothesized a possible role of cdsH in the intestinal phase of the infection. To test this hypothesis, we used an in vitro model of HT-29 cell infection, adding extra cysteine to the culture medium during the infective process. We observed that, at 6 h post-invasion, the wild type S. Typhimurium proliferated 30% more than the ∆cdsH strain in the presence of extra cysteine. This result shows that cdsH contributes to the bacterial replication in the intracellular environment in increased concentrations of extracellular cysteine, strongly suggesting that cdsH participates by increasing the bacterial fitness in the intestinal phase of the S. Typhimurium infection.

Original languageEnglish
Article number2019
Pages (from-to)1-15
Number of pages15
JournalMicroorganisms
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 2020

Keywords

  • CdsH expression
  • Cysteine toxicity
  • Epithelial cell infection

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Microbiology (medical)

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