1. 1. Synthesis of apo A-I in the mucosa of the proximal intestine of the carp could not be measured despite the use of different experimental approaches. Nevertheless, the content of apo A-I in the intestinal tissue was identified by immunocytochemistry. 2. 2. Transepithelial apo A-I transfer from the intestinal lumen into the lamina propria was visualized in tissue sections. Summer-acclimatized fish exhibited a strong intemalization of the apolipoprotein whilst winter-acclimatized carp did not. However, the latter resembled the summer-Actapted fish on insulin treatment. 3. 3. The absorbed apo A-I concentrated in the granular cells of the lamina propria, surrounding the granules, and was released into the systemic circulation probably through blood capillaries. 4. 4. The transfer of undegraded apo A-I into the circulatory system was confirmed by using biotin-labeled carp HDL-apolipoproteins as a probe. 5. 5. The absorptive process responded to thermal Actaptation. During cold-acclimation the transfer process was negligible with respect to the warm-Actapted state. 6. 6. Apo A-I bile content was confirmed. It was suggested that at least part of the HDL used for the transport of lipids proceeds from the bile.
|Number of pages||9|
|Journal||Comparative Biochemistry and Physiology -- Part A: Physiology|
|Publication status||Published - Mar 1992|
ASJC Scopus subject areas