Carp apolipoprotein a-i intestinal absorption and transfer into the systemic circulation during the acclimatization of the carp (Cyprinus carpio)

Maria Ines Vera, Francisco Romero, Rodolfo Amthauer, Jaime Figueroa, Oscar Goicoechea, Gloria Leon, Manuel Krauskopf

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

1. 1. Synthesis of apo A-I in the mucosa of the proximal intestine of the carp could not be measured despite the use of different experimental approaches. Nevertheless, the content of apo A-I in the intestinal tissue was identified by immunocytochemistry. 2. 2. Transepithelial apo A-I transfer from the intestinal lumen into the lamina propria was visualized in tissue sections. Summer-acclimatized fish exhibited a strong intemalization of the apolipoprotein whilst winter-acclimatized carp did not. However, the latter resembled the summer-Actapted fish on insulin treatment. 3. 3. The absorbed apo A-I concentrated in the granular cells of the lamina propria, surrounding the granules, and was released into the systemic circulation probably through blood capillaries. 4. 4. The transfer of undegraded apo A-I into the circulatory system was confirmed by using biotin-labeled carp HDL-apolipoproteins as a probe. 5. 5. The absorptive process responded to thermal Actaptation. During cold-acclimation the transfer process was negligible with respect to the warm-Actapted state. 6. 6. Apo A-I bile content was confirmed. It was suggested that at least part of the HDL used for the transport of lipids proceeds from the bile.

Original languageEnglish
Pages (from-to)573-581
Number of pages9
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume101
Issue number3
DOIs
Publication statusPublished - Mar 1992

ASJC Scopus subject areas

  • Physiology

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