cAMP receptor protein (CRP) positively regulates the yihU-yshA operon in Salmonella enterica serovar Typhi

J. M. Villarreal, I. Hernández-Lucas, F. Gil, I. L. Calderón, E. Calva, C. P. Saavedra

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Salmonella enterica serovar Typhi (S. Typhi) is the aetiological agent of typhoid fever in humans. This bacterium is also able to persist in its host, causing a chronic disease by colonizing the spleen, liver and gallbladder, in the last of which the pathogen forms biofilms in order to survive the bile. Several genetic components, including the yihU-yshA genes, have been suggested to be involved in the survival of Salmonella in the gallbladder. In this work we describe how the yihU-yshA gene cluster forms a transcriptional unit regulated positively by the cAMP receptor global regulator CRP (cAMP receptor protein). The results obtained show that two CRP-binding sites on the regulatory region of the yihU-yshA operon are required to promote transcriptional activation. In this work we also demonstrate that the yihU-yshA transcriptional unit is carbon catabolite-repressed in Salmonella, indicating that it forms part of the CRP regulon in enteric bacteria.

Original languageEnglish
Pages (from-to)636-647
Number of pages12
JournalMicrobiology
Volume157
Issue number3
DOIs
Publication statusPublished - Mar 2011

ASJC Scopus subject areas

  • Microbiology

Fingerprint

Dive into the research topics of 'cAMP receptor protein (CRP) positively regulates the yihU-yshA operon in Salmonella enterica serovar Typhi'. Together they form a unique fingerprint.

Cite this